cat file.csv | perl afterglow.pl -c color.properties > file.dot
while true; do \tcpdump -vttttnneli ath0 | \ ./tcpdump2csv.pl "sip dip dport" | head -2000 | \ ../graph/afterglow.pl -c color.properties -e 2 | neato -Tgif -o test.gif; done
Raffy gave a presentation at DefCon. and one at EuSecWest.
The book, Applied Security Visualization also has a lot of information about AfterGlow.
Have a look at this blog post.